[THC] Free yeast

Mon Sep 22 20:05:20 EDT 2008

THCers:

I will volunteer to maintain the bank if somebody can volunteer some -80
space. I am a brand-new PhD student here so I'll be here for awhile, but I
don't think my advisor would go for a yeast bank in his freezers - there is
already enough eukaryote DNA floating around the lab confusing the
sequencer! Though I've never banked yeast, I have worked in a microbiology
lab, know basic yeast culturing, can do aseptic technique, and have been
brewing for five years or so I think I can handle the technique and can
appreciate the importance of good hygeine.

Oh, and I guess I need to come to a THC meeting. When's the next one?

Cheers,
Steve

On Mon, Sep 22, 2008 at 10:57 AM, stephen baluch <stephenbaluch at yahoo.com>wrote:

> Thanks Linda, after being a college student for 10 years I am definitely
> happy to see that light.
>
> I just emailed Ryan of the Hopjockeys, and told him THC and they were
> interested.  Good news is the whole bank is in duplicate so I will give THC
> one and and they the other.
>
> If you don't use a -80, from what I know, the cultures won't last
> indefinitely.  I cannot give a time frame, but I think as long as its around
> -20 it will last at least 5 years.  The protocol does not change.
>
> This is a must for any club in my opinion.  All it takes is to have a
> couple people trained in the technique, and a little coordination in what
> you all want to brew and you can have enough yeast to never have to buy it
> again.  Even though there are quite a few strains in there, I really only
> used 3.  WY1388 for the Belgians.  Ch'Ti for any lagers (a really great
> strain).  And the mighty Pacman (Rogue Ales) for everything else.
>
> Kick this around, and let me know what you want to do; I will certainly
> help you all get started.
> Stephen
>
> ---------------------------------------------------------------------
> Science is not perfect, it's often misused, it's only a tool, but it's the
> best tool we have, self-correcting, ever changing, applicable to everything.
>  - Carl Sagan
>
>
> --- On Sun, 9/21/08, Linda Swihart <swihart at purdue.edu> wrote:
>
> > From: Linda Swihart <swihart at purdue.edu>
> > Subject: Re: [THC] Free yeast
> > To:
> > Cc: thc at lafinlarry.net
> > Date: Sunday, September 21, 2008, 9:24 PM
> > Hey Stephen -- we're excited about this prospect, at
> > least those of us
> > talking about it at the meeting today are.
> >
> > Jerome forwarded it to the Hop Jockeys list and they are
> > too.  I met a
> > guy down there at an August meeting who is banking a bunch
> > of yeasts,
> > has workplace access and skills that make it a natural for
> > him.
> >
> > What are the pros and cons of keeping it in a regular temp
> > non-front-free freezer, versus a minus 80?  Are there
> > serious ways that
> > things have to be done differently or does the yeast
> > significantly less
> > well preserved?
> >
> > It's hard to believe it's been long enough that
> > you're leaving; seems
> > like you just got here!  (Congratulations on seeing the
> > light at the end
> > of the tunnel!)
> >
> > Linda
> >
> >
> >
> > Thomas Hess wrote:
> > > It will be a shame if it isn't maintained in a -80
> > freezer.
> > >
> > > Tom
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-- 
Steve Kimble
Master's Candidate, Department of Biology
University of Alabama at Birmingham
sjkimble at uab.edu
sjkimble at gmail.com
205.337.4843
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